SPM_005345

Uniprot: A0A037UKZ6
Description: glycerol-3-phosphate dehydrogenase
EC number: 1.1.1.94
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H. {ECO:0000256|HAMAP-Rule:MF_00394, ECO:0000256|RuleBase:RU000439}.
Cofactor:
Enzyme regulation:
Function [CC]:
Pathway: PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism. {ECO:0000256|HAMAP-Rule:MF_00394}.
Active site: ACT_SITE 197 197 Proton acceptor. {ECO:0000256|HAMAP-Rule:MF_00394, ECO:0000256|PIRSR:PIRSR000114-1}.
Binding site: BINDING 111 111 NAD; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00394}.; BINDING 111 111 Substrate. {ECO:0000256|HAMAP-Rule:MF_00394, ECO:0000256|PIRSR:PIRSR000114-2}.; BINDING 146 146 NAD; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00394}.; BINDING 261 261 NAD. {ECO:0000256|HAMAP-Rule:MF_00394}.; BINDING 287 287 NAD. {ECO:0000256|HAMAP-Rule:MF_00394}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 13 18 NAD. {ECO:0000256|HAMAP-Rule:MF_00394}.
Site:
Gene names (primary): gpsA
Gene names (synonym):
Mass: 36,933
Subunit structure [CC]:
Gene ontology (GO): glycerol-3-phosphate dehydrogenase complex [GO:0009331]; glycerol-3-phosphate dehydrogenase [NAD(P)+] activity [GO:0047952]; glycerol-3-phosphate dehydrogenase [NAD+] activity [GO:0004367]; glycerol-3-phosphate dehydrogenase [NADP+] activity [GO:0036439]; NAD binding [GO:0051287]; carbohydrate metabolic process [GO:0005975]; glycerol-3-phosphate biosynthetic process [GO:0046167]; glycerol-3-phosphate catabolic process [GO:0046168]; glycerophospholipid metabolic process [GO:0006650]; phospholipid biosynthetic process [GO:0008654]
Gene ontology IDs: GO:0004367; GO:0005975; GO:0006650; GO:0008654; GO:0009331; GO:0036439; GO:0046167; GO:0046168; GO:0047952; GO:0051287
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family. {ECO:0000256|HAMAP-Rule:MF_00394, ECO:0000256|RuleBase:RU000437, ECO:0000256|SAAS:SAAS00567958}.
Protein families: NAD-dependent glycerol-3-phosphate dehydrogenase family
Coiled coil:
Domain [FT]: DOMAIN 8 163 NAD_Gly3P_dh_N. {ECO:0000259|Pfam:PF01210}.; DOMAIN 186 326 NAD_Gly3P_dh_C. {ECO:0000259|Pfam:PF07479}.
Motif:
Region: REGION 261 262 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00394, ECO:0000256|PIRSR:PIRSR000114-2}.
EMBL: AGBZ02000004
ProteinModelPortal: A0A037UKZ6
MEROPS:
EnsemblBacteria KO: KAI92162
UniPathway:
CDD:
Gene3D:
HAMAP: 1.10.1040.10;3.40.50.720
InterPro: MF_00394
PANTHER: IPR008927;IPR013328;IPR006168;IPR006109;IPR011128;IPR016040
PIRSF:
PRINTS: PIRSF000114
PROSITE: PR00077
Pfam:
ProDom: PF07479;PF01210
SMART:
SUPFAM:
TIGRFAMs: SSF48179;SSF51735

132574-133585(+)

>nucleotide sequence
ATGATGAAAAAAACACAAAAAAATATTACTATTATTGGAACAGGAGCATATGGGACAGTT
TTAGCAAATGTTTTGACAGATAATGATCATAACGTTATTATGTATGGAATTGATAATAAA
GAAGTTGATGATATTAATAATGCACATTTAAATCGTCATTTTTTTGGTAATTTAAAAATT
AATAAAGAAATTAAAGCTACAACAAATTTTGCTGAAGCAGTTGAAGATGCTGAATATATT
ATTTTAGGAATCCCTGTTGTTGCTATTAAATTAATTATTGAAAAGATTAATCAAACAGTA
ACTAAACCAGTTGTTATTATTAATGTTGCTAAAGGATTAGACCCTGACACGCATGAAGTT
TTATCAAAATCAATTATAAAATTAGTGAATCCAAAAATTTTAAAAGCATATGCTGGTATT
TATGGCCCAAGTATTGCAAAAGAAGTTTTACAACGAAAACCAACATGTATTATGGCGGTT
TCACAGGATTTTGCAATTGCACAAGAAGTTCGTGAATTATTTAACAATGAATATTTTGTT
ACTTTTGCTAATACGGATGTTATTGGTACTGAATATGCTGTTGCTTTGAAAAATGCAGTA
GCAATTGCCTCAGGAATATTTAATGGTCTTTATGAATCAGATAACGCTAAAGCATCATTA
ATTACGATGGGATTAAATGAGATTCAACTTTTTGCCAAAACAAAAGGAGCTCAAATTGAA
ACTTTTTTTAACTTTGCGGGATTAGCTGATTTAATTTTAACAACTACCTCAAGCAAATCA
CGAAATTATTCTCTTGGTTTTGAAATTGCTCAAGTTGATAATGCTCAAAAAGTTTTATCA
GAGCAGGTAAAAACAGTAGAAGGCGTTTTAACTTGTAAAACAATTGTGTTAGATGCGCGA
GCAAATAATATTACTCTACCTCTATTTGAAGCTTTATATGATATTTTATATCATAATAAA
CGGCCAAGTGTAATTATTAATAATGTTTTTACAAAAGCAATATTGTCCTAA

>protein sequence
MMKKTQKNITIIGTGAYGTVLANVLTDNDHNVIMYGIDNKEVDDINNAHLNRHFFGNLKI
NKEIKATTNFAEAVEDAEYIILGIPVVAIKLIIEKINQTVTKPVVIINVAKGLDPDTHEV
LSKSIIKLVNPKILKAYAGIYGPSIAKEVLQRKPTCIMAVSQDFAIAQEVRELFNNEYFV
TFANTDVIGTEYAVALKNAVAIASGIFNGLYESDNAKASLITMGLNEIQLFAKTKGAQIE
TFFNFAGLADLILTTTSSKSRNYSLGFEIAQVDNAQKVLSEQVKTVEGVLTCKTIVLDAR
ANNITLPLFEALYDILYHNKRPSVIINNVFTKAILS