SPM_005615

Uniprot: A0A037UNL6
Description: 1-deoxy-D-xylulose 5-phosphate reductoisomerase
EC number: 1.1.1.267
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: 2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH. {ECO:0000256|HAMAP-Rule:MF_00183, ECO:0000256|SAAS:SAAS00313784}.
Cofactor: COFACTOR: Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000256|HAMAP-Rule:MF_00183, ECO:0000256|SAAS:SAAS00313790};
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP). {ECO:0000256|HAMAP-Rule:MF_00183, ECO:0000256|SAAS:SAAS00313787}.
Pathway: PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. {ECO:0000256|HAMAP-Rule:MF_00183, ECO:0000256|SAAS:SAAS00313810}.
Active site:
Binding site: BINDING 123 123 Substrate. {ECO:0000256|HAMAP-Rule:MF_00183}.; BINDING 150 150 Substrate. {ECO:0000256|HAMAP-Rule:MF_00183}.; BINDING 172 172 Substrate. {ECO:0000256|HAMAP-Rule:MF_00183}.; BINDING 195 195 Substrate. {ECO:0000256|HAMAP-Rule:MF_00183}.; BINDING 217 217 Substrate. {ECO:0000256|HAMAP-Rule:MF_00183}.
Calcium binding:
DNA binding:
Metal binding: METAL 148 148 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00183}.; METAL 150 150 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00183}.; METAL 217 217 Divalent metal cation. {ECO:0000256|HAMAP-Rule:MF_00183}.
Nucleotide binding: NP_BIND 7 36 NADP. {ECO:0000256|HAMAP-Rule:MF_00183}.
Site:
Gene names (primary): dxr
Gene names (synonym):
Mass: 43,008
Subunit structure [CC]:
Gene ontology (GO): 1-deoxy-D-xylulose-5-phosphate reductoisomerase activity [GO:0030604]; isomerase activity [GO:0016853]; metal ion binding [GO:0046872]; NADPH binding [GO:0070402]; isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway [GO:0019288]; terpenoid biosynthetic process [GO:0016114]
Gene ontology IDs: GO:0016114; GO:0016853; GO:0019288; GO:0030604; GO:0046872; GO:0070402
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the DXR family. {ECO:0000256|HAMAP-Rule:MF_00183, ECO:0000256|SAAS:SAAS00537059}.
Protein families: DXR family
Coiled coil:
Domain [FT]: DOMAIN 4 130 DXP_reductoisom. {ECO:0000259|Pfam:PF02670}.; DOMAIN 144 225 DXP_redisom_C. {ECO:0000259|Pfam:PF08436}.; DOMAIN 255 370 DXPR_C. {ECO:0000259|Pfam:PF13288}.
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92208
UniPathway:
CDD:
Gene3D:
HAMAP: 3.40.50.720
InterPro: MF_00183
PANTHER: IPR003821;IPR013644;IPR013512;IPR026877;IPR016040
PIRSF: PTHR30525
PRINTS: PIRSF006205
PROSITE:
Pfam:
ProDom: PF08436;PF02670;PF13288
SMART:
SUPFAM:
TIGRFAMs: SSF51735;SSF69055

182385-183531(+)

>nucleotide sequence
ATGCAAAATATTATTATTTTTGGCGCTTCTGGGAATATTGGTCAACAAGCGTTACAAATT
ATTGAATCAAATCCAAATGATTTTCAAATTACAGCTGTTAGTATTTATCATAATGTTCCA
ACTCTAATTAAGATTTTAGAAAAGCATCCCACAATCAGGATTGTTCATCTTGGTGATGAA
ACCCAACAAGCTATTTTAACAGCACAATATCCAAATATAACTTTTGTAACCGGAGAAACG
GGAATTAATGCAATGTTAGCTGCTTTTCCTACAGCTTTAGTTTTAAATGCAATTAGTGGT
TTTGCAGGATTATACCCAACATTACAAACTTTATATGGAAAAAATCGTACTTTATTATTA
GCAAATAAAGAATCATTAGTAGTGGCAGGGGATTTAATTAACAGTTTATTAACAAAAAAT
AATAATCAATTATATCCAATTGATTCTGAACATTGTGCTATTTTTCAATGTTTAGAGCAA
ACCAATCCGTGTTCAGAAATTATTTTAACAGCTTCGGGAGGGATGTTTGCTAATAAAACA
TTAACTGATTTAAAAACGATTGATGAGCAACAAGCATTACAACATCCAACTTGAAAGATG
GGACAAAATATTACCATTGATTCTTCAACAATGGTGAACAAAGGATTGGAAATTATTGAA
GCTTATCATTTATTTAAAACTTCGAAAATTACTGTTGTTTTACATCCACAAGCTATTTTA
CATTCTGCCGTACAATATGCAGATTATTCAATTATTGGCCAATTATCAAAGCCTTCAATG
TTACAAGTTTTAAATTATTTTTTATATTATCCAACTCGAAAGAATAGTTCTTTCTTAAAA
CCATTAAATTTTGAGGAATTAATAACTTTAACATTTCAAAAAGCTGATTTATCACGTTGA
AAAGCACTACAGTTAGCCTATCGTTGTTTGAATGAAAATAATTCATTAGCAATTGCTTTT
AATGCAGCAAACGAAGAATTACGTTCTCTTTTTGTAGTTGGAAAAATTAAGTTTTATCAA
ATTGTGGATTATATTGAATATTTTATGGACCAAATTAAGCCACAAAAATTAGTTAATTAT
CGTGAAATCAAAGAATTAAATGATATCATTAAAAGAGATATTATTAATTATTTTTCTGAA
AAATAA

>protein sequence
MQNIIIFGASGNIGQQALQIIESNPNDFQITAVSIYHNVPTLIKILEKHPTIRIVHLGDE
TQQAILTAQYPNITFVTGETGINAMLAAFPTALVLNAISGFAGLYPTLQTLYGKNRTLLL
ANKESLVVAGDLINSLLTKNNNQLYPIDSEHCAIFQCLEQTNPCSEIILTASGGMFANKT
LTDLKTIDEQQALQHPTWKMGQNITIDSSTMVNKGLEIIEAYHLFKTSKITVVLHPQAIL
HSAVQYADYSIIGQLSKPSMLQVLNYFLYYPTRKNSSFLKPLNFEELITLTFQKADLSRW
KALQLAYRCLNENNSLAIAFNAANEELRSLFVVGKIKFYQIVDYIEYFMDQIKPQKLVNY
REIKELNDIIKRDIINYFSEK