SPM_005640

Uniprot: A0A037UNM1
Description: glycyl-tRNA ligase
EC number: 6.1.1.14
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00015999}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the attachment of glycine to tRNA(Gly). {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00345537}.
Pathway:
Active site:
Binding site: BINDING 98 98 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.; BINDING 168 168 Substrate. {ECO:0000256|HAMAP-Rule:MF_00253}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 200 202 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 210 215 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 284 285 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.; NP_BIND 328 331 ATP. {ECO:0000256|HAMAP-Rule:MF_00253}.
Site:
Gene names (primary): glyQS
Gene names (synonym):
Mass: 53,107
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00006558}.
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; glycine-tRNA ligase activity [GO:0004820]; glycyl-tRNA aminoacylation [GO:0006426]
Gene ontology IDs: GO:0004820; GO:0005524; GO:0005737; GO:0006426
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family. {ECO:0000256|HAMAP-Rule:MF_00253, ECO:0000256|SAAS:SAAS00541124}.
Protein families: Class-II aminoacyl-tRNA synthetase family
Coiled coil:
Domain [FT]: DOMAIN 146 363 AA_TRNA_LIGASE_II. {ECO:0000259|PROSITE:PS50862}.
Motif:
Region: REGION 215 219 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.; REGION 324 328 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_00253}.
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92213
UniPathway:
CDD:
Gene3D: cd00774
HAMAP: 3.40.50.800
InterPro: MF_00253_B
PANTHER: IPR002314;IPR006195;IPR004154;IPR027031;IPR022961;IPR033731;IPR002315
PIRSF: PTHR10745
PRINTS:
PROSITE: PR01043
Pfam: PS50862
ProDom: PF03129;PF00587
SMART:
SUPFAM:
TIGRFAMs: SSF52954

191058-192426(+)

>nucleotide sequence
ATGCAATATACATTAGAAGAAGTTGTTAATCATTTAAAAACACAAGGTTTTGTGTTTCAA
GGTAGTGAGATTTATGGCGGTTTAGCAAACAGCTGAGATTTTGGACCGTTGGGGGTTGAA
TTAGAACGAAATTTAAAAAATTTATGATGACAACATTTTATTACGAAATCAAAATATAAT
GTTGGTCTTGATAGTGCTATTTTAATGAACAATAATGTTTGAAAAGCTTCTGGGCACCTT
GGTAATTTTGCTGACCCATTATTAGACTGTAAAAAGTGTAAAACCCGAATGCGGGCAGAT
AAACTGATTGAAGATAATTATCCGAAATTAAATTGTGGTGGTTGAACTAATGAACAATTA
GAAACTTTTATTACTGAAAAAAATATTTTATGTCCAAATTGTGGTGGACATGACTTTACT
GAAATTCGTCAGTTTGAATTAATGTTTAAAACAAATCAAGGTGTAATTGAAGATGAAAAA
TCAGTTGTTTATTTGCGGCCAGAAACAGCACAAGGAATTTTTGTCAATTTTAAAAATGTC
CAACGTTCTTTACGAAAAAAAATCCCTTTTGGAGTTGGTCAAATTGGAAAGTCATTTCGT
AATGAAATTACCCCGGGAAATTTTATTTTTCGTACACGTGAATTTGAACAAATGGAATTA
GAATTTTTTTATGATAGTAACGAAAAGGTTGATTGATTTGAATATTGAGTTAAAGAAGTA
ACCAAGTTTTTAGAATTAATTAATTTAAAATCAGAAAACTATCGTTTTCGTGAGCATAAT
GCTGATGAATTAGCCCATTATGCGAAACGAACTATTGATATTGAATATAAATTTCCGTTT
GGAATAGGTGAATTATGGGGGATCGCTGACCGTGGGGATTATGATTTACGTCGTCATAGT
GAAATGAGTAAAAATGATTTGTCGTATTTAAATCAAGAAACAAATGAAAAAATTATGCCG
CATGTAATTGAACCATCTGTTGGAGTTGGTCGTTTGCTGCTAGCTATTTTATATGATGCT
TACCATGTTGAAAAAGTTGATGACAATGAGACGAGAATTGTTTTAAAATTAAGTCCGTTA
TTAGCTCCGTATCAAATTGCGGTGATTCCATTAAGCAAACAATTAAACAGCGAGGCTTAT
CAACTATATGAAAAACTCTTAGTTCATTTTCAGTGTACTTATGATGAAACAGGAAATATT
GGAAAGCGTTATCGTCGTCAAGATGCGATTGGAACACCGTTTTGTGTAACAGTTGATTTT
GATACTCAAGAAGACCAAAAAGTTACCGTTCGTAATCGTGATACAATGAAACAAGAACGA
GTGGCAATTACTAATTTAGAAAGTTATTTCACCGCAGCATTAAAATAA

>protein sequence
MQYTLEEVVNHLKTQGFVFQGSEIYGGLANSWDFGPLGVELERNLKNLWWQHFITKSKYN
VGLDSAILMNNNVWKASGHLGNFADPLLDCKKCKTRMRADKLIEDNYPKLNCGGWTNEQL
ETFITEKNILCPNCGGHDFTEIRQFELMFKTNQGVIEDEKSVVYLRPETAQGIFVNFKNV
QRSLRKKIPFGVGQIGKSFRNEITPGNFIFRTREFEQMELEFFYDSNEKVDWFEYWVKEV
TKFLELINLKSENYRFREHNADELAHYAKRTIDIEYKFPFGIGELWGIADRGDYDLRRHS
EMSKNDLSYLNQETNEKIMPHVIEPSVGVGRLLLAILYDAYHVEKVDDNETRIVLKLSPL
LAPYQIAVIPLSKQLNSEAYQLYEKLLVHFQCTYDETGNIGKRYRRQDAIGTPFCVTVDF
DTQEDQKVTVRNRDTMKQERVAITNLESYFTAALK