SPM_006085

Uniprot: A0A037UNV5
Description: nucleoside-triphosphate diphosphatase
EC number: 3.6.1.19
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate. {ECO:0000256|HAMAP-Rule:MF_01405, ECO:0000256|SAAS:SAAS00020483}.
Cofactor: COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-Rule:MF_01405}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|HAMAP-Rule:MF_01405}; ; Note=Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+). {ECO:0000256|HAMAP-Rule:MF_01405};; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000256|SAAS:SAAS00608136};
Enzyme regulation:
Function [CC]: FUNCTION: Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions. {ECO:0000256|HAMAP-Rule:MF_01405}.
Pathway:
Active site:
Binding site: BINDING 156 156 Substrate. {ECO:0000256|HAMAP-Rule:MF_01405}.; BINDING 176 176 Substrate. {ECO:0000256|HAMAP-Rule:MF_01405}.; BINDING 182 182 Substrate. {ECO:0000256|HAMAP-Rule:MF_01405}.
Calcium binding:
DNA binding:
Metal binding: METAL 71 71 Magnesium or manganese. {ECO:0000256|HAMAP-Rule:MF_01405}.
Nucleotide binding:
Site:
Gene names (primary):
Gene names (synonym):
Mass: 23,336
Subunit structure [CC]: SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01405, ECO:0000256|SAAS:SAAS00020467}.
Gene ontology (GO): metal ion binding [GO:0046872]; nucleoside-triphosphatase activity [GO:0017111]; nucleoside-triphosphate diphosphatase activity [GO:0047429]; nucleotide binding [GO:0000166]; nucleoside triphosphate catabolic process [GO:0009143]; purine nucleotide metabolic process [GO:0006163]
Gene ontology IDs: GO:0000166; GO:0006163; GO:0009143; GO:0017111; GO:0046872; GO:0047429
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the HAM1 NTPase family. {ECO:0000256|HAMAP-Rule:MF_01405, ECO:0000256|RuleBase:RU003781, ECO:0000256|SAAS:SAAS00542593}.
Protein families: HAM1 NTPase family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 8 13 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_01405}.; REGION 71 72 Substrate binding. {ECO:0000256|HAMAP-Rule:MF_01405}.
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92283
UniPathway:
CDD:
Gene3D: cd00515
HAMAP: 3.90.950.10
InterPro: MF_01405
PANTHER: IPR002637;IPR029001;IPR020922
PIRSF: PTHR11067
PRINTS:
PROSITE:
Pfam:
ProDom: PF01725
SMART:
SUPFAM:
TIGRFAMs: SSF52972

276370-276985(+)

>nucleotide sequence
ATGGAAGAAATTTGGATTGCTACTAGTAATAAGAACAAAGTACGAGAATTTAAAGAAATG
TTTGAAGGTATTAATATAATTGTTAAATCATTATTAGATTTAACAACACCTGTGCCTGAA
ATTCCTGAAACAGGAACAACCTTTGAAGAAAATGCATTTTTAAAAGCTGATTATTTAAGT
AAAATGTTAAATAAGCCAGTATTAGCGGATGATTCTGGGTTAGAAATTATTGGTTTAGGA
AATTTTCCTGGTGTTAATACTCGCCGTTGAGCAGAACCAATTACTGATAATAATATTATT
AATAATTTATTAATTGAAAAATGTCGGTCACTAGAACAACGTGATGCACAAGCAGTTTGT
GTTTTATGTTATATTAATCCAATTACAAACGAAACAAGATATTTCCGTGGAGTAACAAAA
GGCCTTATTACTGAAGAACCAAGTGGTACTAATGTTTTTGGTTATGATGCCATTTTTTTC
TTACCTGAAATTGGCCAAACTTATGCTGAATTAACTATAATAGAAAAAAATAAGTATTCA
CATCGTTCAAAGGCTTTTCAGATGTTTAAAAAATGATGATTAGGAGGAAAAGATGAAAAC
AACAAAAAAAATTAA

>protein sequence
MEEIWIATSNKNKVREFKEMFEGINIIVKSLLDLTTPVPEIPETGTTFEENAFLKADYLS
KMLNKPVLADDSGLEIIGLGNFPGVNTRRWAEPITDNNIINNLLIEKCRSLEQRDAQAVC
VLCYINPITNETRYFRGVTKGLITEEPSGTNVFGYDAIFFLPEIGQTYAELTIIEKNKYS
HRSKAFQMFKKWWLGGKDENNKKN