SPM_006180

Uniprot: A0A037UP03
Description: thiouridylase
EC number: 2.8.1.13
Annotation score: 3 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor. {ECO:0000256|HAMAP-Rule:MF_00144}.
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. {ECO:0000256|HAMAP-Rule:MF_00144}.
Pathway:
Active site: ACT_SITE 108 108 Nucleophile. {ECO:0000256|HAMAP-Rule:MF_00144}.; ACT_SITE 207 207 Cysteine persulfide intermediate. {ECO:0000256|HAMAP-Rule:MF_00144}.
Binding site: BINDING 33 33 ATP; via amide nitrogen and carbonyl oxygen. {ECO:0000256|HAMAP-Rule:MF_00144}.; BINDING 133 133 ATP; via amide nitrogen. {ECO:0000256|HAMAP-Rule:MF_00144}.
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding: NP_BIND 7 14 ATP. {ECO:0000256|HAMAP-Rule:MF_00144}.
Site: SITE 134 134 Interaction with tRNA. {ECO:0000256|HAMAP-Rule:MF_00144}.; SITE 348 348 Interaction with tRNA. {ECO:0000256|HAMAP-Rule:MF_00144}.
Gene names (primary): mnmA
Gene names (synonym):
Mass: 43,503
Subunit structure [CC]:
Gene ontology (GO): cytoplasm [GO:0005737]; ATP binding [GO:0005524]; sulfurtransferase activity [GO:0016783]; tRNA binding [GO:0000049]; tRNA modification [GO:0006400]
Gene ontology IDs: GO:0000049; GO:0005524; GO:0005737; GO:0006400; GO:0016783
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the MnmA/TRMU family. {ECO:0000256|HAMAP-Rule:MF_00144}.
Protein families: MnmA/TRMU family
Coiled coil:
Domain [FT]:
Motif:
Region: REGION 103 105 Interaction with target base in tRNA. {ECO:0000256|HAMAP-Rule:MF_00144}.; REGION 157 159 Interaction with tRNA. {ECO:0000256|HAMAP-Rule:MF_00144}.; REGION 315 316 Interaction with tRNA. {ECO:0000256|HAMAP-Rule:MF_00144}.
EMBL: AGBZ02000004
ProteinModelPortal: A0A037UP03
MEROPS:
EnsemblBacteria KO: KAI92296
UniPathway:
CDD:
Gene3D:
HAMAP: 2.30.30.280;3.40.50.620
InterPro: MF_00144
PANTHER: IPR023382;IPR014729;IPR004506
PIRSF: PTHR11933
PRINTS:
PROSITE:
Pfam:
ProDom:
SMART:
SUPFAM:
TIGRFAMs:

297559-298693(+)

>nucleotide sequence
ATGAAAAAAGTTGTTGTTGGATTATCTGGGGGAGTTGATTCTTCAGTTAGTTTATATTTA
TTACAACAAGCAGGTTATGATGTTGAAGGTCTTTTTATGCGTAATTGGGACAGTCAATTA
AATAATGATATTTTAGGGAATAAAGCAATTAATAATATGATTTGCCCACAAGAACTTGAT
TATAATGATGCTGTTAATGTTAGTAAAACATTACAAGTGCCATTGCACCGTGTTGATTTT
ATTAAAGAATATTGAGAATATGTTTTTAAACATTTTATTAGTGAATATCAAAAGGGGCGA
ACTCCTAATCCAGATATTTTATGTAATAAATATATTAAATTTGATTATTTTTTAAACTAT
GCAATTAATAATTATCATGCTGATTTTATTGCAATGGGACATTATGCGCGGGTACGTTTC
AATGAAGAATTACAAGAATATCAATTATTACGGGGAATTGACCGAGATAAAGATCAAACT
TATTTCTTAAGTCAGTTGAACCAAGGACAATTATCAAAAACATTTTTTCCGTTAGGAAAT
TTAACAAAAAAAGAAGTTAGAGAAATTGCAATGACGCAAAATTTAAGCACGGCTAATAAA
AAAGATTCAACAGGGATTTGTTTTATTGGCGAACGTGATTTTAAAAATTTTTTACAAAAT
TATATTCCAAATCAAGATGGTAATATTGTTGATATTGAAACAAAAGAAGTTGTTGGTCAT
CATAATGGGGTGATGTATTATACAATTGGTCAACGGCGTGGTTTAAATTTAGGTGGGATG
AGTGAACCATATTTTGTTGCTGGTAAAAATGTTGAAAAAAATATTTTATATGTTGCAAAA
AGTAGCGAAGAAAAATGACTATATTCAACTAGTTGTCTTGTTACTGATGTTAATTGAATT
AATACTTTCCGTGAAAAAGAATTTAATTGTACAGCTAAGTTTCGTTATCGTCAAAAAGAT
ATTCCTGTTAAAATAACCGTTTTAAATGATAACAAATGTCTTGTGGAATTTGCAACAACA
GTAAAAGCAATTACACCAGGACAAGCTGCTGTTTTTTATGTTGATGAAGTTTGTCTTGGC
GGTGGAGTTATTAATGATGTTTATTTAGATAATCAAAAATTATGATATTTATAG

>protein sequence
MKKVVVGLSGGVDSSVSLYLLQQAGYDVEGLFMRNWDSQLNNDILGNKAINNMICPQELD
YNDAVNVSKTLQVPLHRVDFIKEYWEYVFKHFISEYQKGRTPNPDILCNKYIKFDYFLNY
AINNYHADFIAMGHYARVRFNEELQEYQLLRGIDRDKDQTYFLSQLNQGQLSKTFFPLGN
LTKKEVREIAMTQNLSTANKKDSTGICFIGERDFKNFLQNYIPNQDGNIVDIETKEVVGH
HNGVMYYTIGQRRGLNLGGMSEPYFVAGKNVEKNILYVAKSSEEKWLYSTSCLVTDVNWI
NTFREKEFNCTAKFRYRQKDIPVKITVLNDNKCLVEFATTVKAITPGQAAVFYVDEVCLG
GGVINDVYLDNQKLWYL