SPM_006355

Uniprot: A0A037UNZ2
Description: transcription elongation factor GreA
EC number:
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity:
Cofactor:
Enzyme regulation:
Function [CC]: FUNCTION: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides. {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556, ECO:0000256|SAAS:SAAS00338401}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding:
Nucleotide binding:
Site:
Gene names (primary): greA
Gene names (synonym):
Mass: 17,402
Subunit structure [CC]:
Gene ontology (GO): DNA binding [GO:0003677]; translation elongation factor activity [GO:0003746]; regulation of DNA-templated transcription, elongation [GO:0032784]; transcription, DNA-templated [GO:0006351]
Gene ontology IDs: GO:0003677; GO:0003746; GO:0006351; GO:0032784
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the GreA/GreB family. {ECO:0000256|HAMAP-Rule:MF_00105, ECO:0000256|RuleBase:RU000556, ECO:0000256|SAAS:SAAS00541981}.
Protein families: GreA/GreB family
Coiled coil: COILED 49 69 {ECO:0000256|HAMAP-Rule:MF_00105}.
Domain [FT]: DOMAIN 5 74 GreA_GreB_N. {ECO:0000259|Pfam:PF03449}.; DOMAIN 84 156 GreA_GreB. {ECO:0000259|Pfam:PF01272}.
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92328
UniPathway:
CDD:
Gene3D:
HAMAP: 1.10.287.180;3.10.50.30
InterPro: MF_00105
PANTHER: IPR018151;IPR006359;IPR028624;IPR001437;IPR023459;IPR022691
PIRSF:
PRINTS: PIRSF006092
PROSITE:
Pfam: PS00829;PS00830
ProDom: PF01272;PF03449
SMART:
SUPFAM:
TIGRFAMs: SSF46557

342157-342628(+)

>nucleotide sequence
ATGAATGAAGAAATTTTGTTAACTAAAGAGGGAATTAAAGACTTACAAGAAGAATTAGAT
AATTTAATTAATGTTATTCGTCCCGAAGTGATTGAAGAGTTAAAAGAAGCTCGTGCACAG
GGAGACTTATCAGAAAATGCTGATTATGATGCAGCACGAAACCGTCAAGCGGAAGTTGAA
GGACGAATTAAAGAAATTGAATCATTATTAACAAAAGCAAAAGAAATTAAGGAAGTTAAA
TCAAAAACAGGAATTATTAAGTTAGGAAGTACAGTAACGTTTACTAATTTATTAATTAAT
AAAAAATATGAACTTAAAATTGTTGGTGCTGTTGAGGCTAATCCTTTTGAAAATACAATT
TCAAATGAATCACCAATTGCAAAAGCTATTATTGGGAAAAAAGCTGGCGATTTAATTGAA
ATTAAGGGAATTCAAGAACCTTATAAAGTTAAAATTTTAACTGTAGAATAA

>protein sequence
MNEEILLTKEGIKDLQEELDNLINVIRPEVIEELKEARAQGDLSENADYDAARNRQAEVE
GRIKEIESLLTKAKEIKEVKSKTGIIKLGSTVTFTNLLINKKYELKIVGAVEANPFENTI
SNESPIAKAIIGKKAGDLIEIKGIQEPYKVKILTVE