SPM_006395

Uniprot: A0A037UP38
Description: endonuclease IV
EC number: 3.1.21.2
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products. {ECO:0000256|HAMAP-Rule:MF_00152}.
Cofactor: COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-Rule:MF_00152}; ; Note=Binds 3 Zn(2+) ions. {ECO:0000256|HAMAP-Rule:MF_00152}
Enzyme regulation:
Function [CC]: FUNCTION: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin. {ECO:0000256|HAMAP-Rule:MF_00152}.
Pathway:
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 71 71 Zinc 1. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 112 112 Zinc 1. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 147 147 Zinc 1. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 147 147 Zinc 2. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 181 181 Zinc 2. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 184 184 Zinc 3. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 216 216 Zinc 2. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 229 229 Zinc 3. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 231 231 Zinc 3. {ECO:0000256|HAMAP-Rule:MF_00152}.; METAL 261 261 Zinc 2. {ECO:0000256|HAMAP-Rule:MF_00152}.
Nucleotide binding:
Site:
Gene names (primary): nfo
Gene names (synonym):
Mass: 34,063
Subunit structure [CC]:
Gene ontology (GO): deoxyribonuclease IV (phage-T4-induced) activity [GO:0008833]; DNA binding [GO:0003677]; zinc ion binding [GO:0008270]; DNA repair [GO:0006281]
Gene ontology IDs: GO:0003677; GO:0006281; GO:0008270; GO:0008833
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the AP endonuclease 2 family. {ECO:0000256|HAMAP-Rule:MF_00152, ECO:0000256|SAAS:SAAS00546595}.
Protein families: AP endonuclease 2 family
Coiled coil:
Domain [FT]: DOMAIN 29 238 AP_endonuc_2. {ECO:0000259|Pfam:PF01261}.
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92336
UniPathway:
CDD:
Gene3D:
HAMAP: 3.20.20.150
InterPro: MF_00152
PANTHER: IPR001719;IPR018246;IPR013022
PIRSF: PTHR21445
PRINTS:
PROSITE:
Pfam: PS00729;PS00731;PS51432
ProDom: PF01261
SMART:
SUPFAM: SM00518
TIGRFAMs: SSF51658

349470-350382(+)

>nucleotide sequence
TTAATTATTGGGAGTCACGTTAGTATGAACAAGCAGCAAAATTATTTGCTGGGTGCCTTA
AATGAAAGTCTAAATAATAATGCTAACGCAATGATGATATATACAGGACCACCCCAAAAT
ACTATTCGTGTTAGTACTGAACAATTTTTTATTCCAGAATTTTATCAACAATTAAAAGAA
CATCATTTTGATATTAATAATATTATTATTCATGCTCCTTATATTATTAATTTAGCGAAC
ACAACTAATCCTAGTACTTTTGAAATAGCTGTTGAGTTTTTAAAAAAAGAAATTACTCGT
GCTGATGAAATTGGAATTAAAACAATTGTTTTACATCCTGGAAGTGGTGTTGGCGCGCCA
GAACAAGTTGGATTAGATCGAATTATTGAAGGATTAAATTTAGTTTTAACACCTAATCAA
AAAGCAAAAATTGCTTTAGAAACAATGGCTGGCAAGGGGAGCGAATTAGGAACAAGTTTT
GCCCAACTAAAATATATTATTGATAATGTTAAATTAAATGATAAATTAGGGGTTTGTTGA
GATACTTGTCATATGCATGATGCTGGTTATCGCTTGACAAAAGCGTTAGATGATATTATT
ATAGAATTTGAGCAATTAATTGGTTTAAACCGGTTATTATGTTTACATATTAATGACAGT
AAAAATCCTTTAAATGCCCATAAGGATCGCCACGAAAACATTGGCTATGGTTACTTGGGA
TTTGAAACCCTTTTAAAAATAATTTATCATCCTAAATTAGATGGGATGATTAAGATTTTA
GAAACACCATATGTTGGAGAAAAGAAGCATGCGGTTGCTCCTTATAAGGATGAAATTGCA
ATGATTAAGGCAAAAAATTTTACTGACCCATTTCAACAAAAGGGTAAAATAAAAATAGAT
TTAGGAGAATAA

>protein sequence
LIIGSHVSMNKQQNYLLGALNESLNNNANAMMIYTGPPQNTIRVSTEQFFIPEFYQQLKE
HHFDINNIIIHAPYIINLANTTNPSTFEIAVEFLKKEITRADEIGIKTIVLHPGSGVGAP
EQVGLDRIIEGLNLVLTPNQKAKIALETMAGKGSELGTSFAQLKYIIDNVKLNDKLGVCW
DTCHMHDAGYRLTKALDDIIIEFEQLIGLNRLLCLHINDSKNPLNAHKDRHENIGYGYLG
FETLLKIIYHPKLDGMIKILETPYVGEKKHAVAPYKDEIAMIKAKNFTDPFQQKGKIKID
LGE