SPM_006405

Uniprot: A0A037UP01
Description: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
EC number: 1.17.7.3
Annotation score: 2 out of 5
Miscellaneous [CC]:
Protein existence: Inferred from homology
Catalytic activity: CATALYTIC ACTIVITY: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin. {ECO:0000256|HAMAP-Rule:MF_00159}.
Cofactor: COFACTOR: Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000256|HAMAP-Rule:MF_00159}; ; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|HAMAP-Rule:MF_00159}
Enzyme regulation:
Function [CC]: FUNCTION: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. {ECO:0000256|HAMAP-Rule:MF_00159, ECO:0000256|SAAS:SAAS00571352}.
Pathway: PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6. {ECO:0000256|HAMAP-Rule:MF_00159, ECO:0000256|SAAS:SAAS00088771}.
Active site:
Binding site:
Calcium binding:
DNA binding:
Metal binding: METAL 264 264 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-Rule:MF_00159}.; METAL 267 267 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-Rule:MF_00159}.; METAL 299 299 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-Rule:MF_00159}.; METAL 306 306 Iron-sulfur (4Fe-4S). {ECO:0000256|HAMAP-Rule:MF_00159}.
Nucleotide binding:
Site:
Gene names (primary): ispG
Gene names (synonym):
Mass: 39,740
Subunit structure [CC]:
Gene ontology (GO): 4 iron, 4 sulfur cluster binding [GO:0051539]; 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity [GO:0046429]; iron ion binding [GO:0005506]; isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway [GO:0019288]; terpenoid biosynthetic process [GO:0016114]
Gene ontology IDs: GO:0005506; GO:0016114; GO:0019288; GO:0046429; GO:0051539
Chain:
Signal peptide:
Domain [CC]:
Sequence similarities: SIMILARITY: Belongs to the IspG family. {ECO:0000256|HAMAP-Rule:MF_00159, ECO:0000256|SAAS:SAAS00571349}.
Protein families: IspG family
Coiled coil:
Domain [FT]:
Motif:
Region:
EMBL: AGBZ02000004
ProteinModelPortal:
MEROPS:
EnsemblBacteria KO: KAI92338
UniPathway:
CDD:
Gene3D:
HAMAP:
InterPro: MF_00159
PANTHER: IPR011005;IPR016425;IPR004588
PIRSF:
PRINTS: PIRSF004640
PROSITE:
Pfam:
ProDom: PF04551
SMART:
SUPFAM:
TIGRFAMs: SSF51717

351085-352168(-)

>nucleotide sequence
TTATTGCTGAATGTTTTTTTGTTGTCATTCTTGATAATATTCATCAATTAATAGTTTTAA
TTCTGGAACAAGATCTTCTTGTTTACATGATTTATAAATTTTACCTTTTTTAAAAATAAT
TCCACCATTTTTACCACCAGCAATTCCTAAATCAGCTTGTTTAGCTTCTCCAGGACCATT
AACAACACAACCTAAAATGGCAATTTTTAATGGAATTTTTAAATCTTTTGTATATTCTTC
TAATTCTTTTACAACAGGGAAAAGGTCATATTCTAAACGACCACATGTTGGACAAGCAAT
CACATCAACAATGTTATCATAAAGTCCTAGTGAATTTAACATTCGTTTAGCAACTTCAAC
TTCGGCGATTGGATCAGTTGATAAACTAATCCGAATCGTATCACCAATGCCATTAAAAAG
GAGCGGTGATAAACCACTACATGATTTAATTGTTCCTGTATGATGACTGCCAGCTTCAGT
AATTCCTAAATGAAGGGGATAATTTCATTCTTGGCTAGCTAAGGTATAAGCTTCAATTGC
CATTAATGGTTCAGTTGCTTTTAAAGATAAAATAATATCATAAAAACCAAGGTGCTCTAA
AATTTCAATATGACGGCGAGCACTTTCAATCATTGCTTTAGGAGTTCAACCATATTTATT
AACAAGATCTAATGGTAAACTACCAGAATTAACCCCAATTCGAATTGGAATCTTTTTTTC
AAGACATTTTTCAACAACTTTTTTTGTATTTTCTATACTTCCAATGTTACCTGGATTAAT
TCGAATTTTACTAATACCAGCATCAGCAGCGATTAAAGCAAATTCATAATTAAAATGGAT
GTCCGCAACTAATGGGCAAGGAGAACGTTCAACAATTGTTTTTAAGACAGCAGCATCATC
TTTTCCTAATACAGCAATTCGAATAATTTCACAACCTTCTTGATATAGTTGATTAATTTG
TGATAAAGTAGCTTCAACATCGTGTGTTTTCGTATTAGTCATTGATTGAATAACAACTTT
ATTTTGGCCGCCAATTTGAACATTACCAACCATTACTTTTCGAGTATTATTTCGTTTATT
CAT

>protein sequence
MNKRNNTRKVMVGNVQIGGQNKVVIQSMTNTKTHDVEATLSQINQLYQEGCEIIRIAVLG
KDDAAVLKTIVERSPCPLVADIHFNYEFALIAADAGISKIRINPGNIGSIENTKKVVEKC
LEKKIPIRIGVNSGSLPLDLVNKYGWTPKAMIESARRHIEILEHLGFYDIILSLKATEPL
MAIEAYTLASQEWNYPLHLGITEAGSHHTGTIKSCSGLSPLLFNGIGDTIRISLSTDPIA
EVEVAKRMLNSLGLYDNIVDVIACPTCGRLEYDLFPVVKELEEYTKDLKIPLKIAILGCV
VNGPGEAKQADLGIAGGKNGGIIFKKGKIYKSCKQEDLVPELKLLIDEYYQEWQQKNIQQ