TSSs exact mapping at single-nucleotide resolution and quantification of promoter activity
We developed a new procedure for RNA 5′-end enrichment (5`-ERS), that allows precisely identify the coordinates of transcription start sites (TSSs) at single-nucleotide resolution. We applied our method to exactly map TSSs in three Mollicutes species.
Scheme of 5`-ERS method
Transcription factors distribution across Mollicutes
For 50 Mollicute species from Mycoplasma, Spiroplasma, Acholeplasma amd Phytoplasma groups we identified and compared all transcription factors. Phylogenetic tree was constructed based on alignments of orthologs conserved between all species
Reconstruction of transcription regulation networks
Using data about TSS positions with comparative genomics methods we predicted binding sites for about a half trascription factors in Mycoplasma, Spiroplasma and Acholeplasma and predicted targets of that factors.