TSSs exact mapping at single-nucleotide resolution and quantification of promoter activity

We developed a new procedure for RNA 5′-end enrichment (5`-ERS), that allows precisely identify the coordinates of transcription start sites (TSSs) at single-nucleotide resolution. We applied our method to exactly map TSSs in three Mollicutes species.

Scheme of 5`-ERS method

Download table with TSSs positions and promoter activities in control and heatshock states

Transcription factors distribution across Mollicutes

For 50 Mollicute species from Mycoplasma, Spiroplasma, Acholeplasma amd Phytoplasma groups we identified and compared all transcription factors. Phylogenetic tree was constructed based on alignments of orthologs conserved between all species

Reconstruction of transcription regulation networks

Using data about TSS positions with comparative genomics methods we predicted binding sites for about a half trascription factors in Mycoplasma, Spiroplasma and Acholeplasma and predicted targets of that factors.

Download table with transcription factors, their functions and binding sites

Download file with the alignments of transcription binding sites

Transcriptional regulons of A.laidlawii

Transcriptional regulons of S.melliferum (A) and M.gallisepticum (B)

© Fisunov Lab of Proteomics, 2016-2018